#Zetasizer nano series cuvettes upgrade

NOTE: All systems with S/N less than MAL500119 need an upgrade to the optics. RAFT SMALPs show promise as a new membrane mimetic with different nanoscale sizes, which can be used for a wide variety of biophysical studies of membrane proteins. For size and molecular weight measurements for use with the Zetasizer Nano series (Minimum sample volume S/ZS is 12 microlitres, S90/ZS90 minimum is 20 microlitres, maximum volume 45 microlitres).

Due to the polymer acting as a surfactant that forms disc like nanoparticles, we term these StMA based block copolymers “RAFT SMALPs”. At a weight ratio of 1.25:1 StMA to lipid, the nanoparticle size created is 28 + 1 nm for a 2:1 ratio, 10 + 1 nm for a 3:1 StMA ratio and 32 + 1 nm for a 4:1 StMA ratio independent of the molecular weight of the polymer. A combination of dynamic light scattering (DLS), solid-state nuclear magnetic resonance (SSNMR) spectroscopy, and transmission electron microscopy (TEM) was used to characterize the size of the nanoparticles created using these StMA polymers. The new Zetasizer Nano ZS from Malvern Panalytical brings the practicality of a maintenance-free system with the versatility to offer precision measurement for your laboratory's particle. These polymers act as macromolecular surfactants for 1-Palmitoyl-2-oleoyl- sn-glycero-3-phosphocholine (POPC)/1-palmitoyl-2-oleoyl- sn-glycero-3-phospho-(1′- rac-glycerol (POPG) lipids, forming disc like structures of the lipids with the polymer wrapping around the hydrophobic lipid edge. The Zetasizer Advance family is the most versatile and flexible range of light scattering systems on the market, with a solution for every challenge and every budget. StMA polymers with different compositions and molecular weights were synthesized and characterized by size exclusion chromatography (SEC). Here we report the controlled synthesis and characterization of StMA containing block copolymers. Recently, styrene-maleic acid (StMA) copolymers have been shown to facilitate the formation of disc-like lipid bilayer mimetics that maintain the structural and dynamic integrity of membrane proteins. Characterization of membrane proteins is challenging due to the difficulty in mimicking the native lipid bilayer with properly folded and functional membrane proteins.

Zetasizer nano series cuvettes